簡化您的PCR反應，ABM提供新的方法與試劑成分，原本無法直接由血液反應PCR的作法，現在提供Blooddirect 2X PCRMasterMix反應試劑組。
該方法簡化了抽gDNA的過程，簡化了PCR工作流程，ABM提供各種高度敏感MasterMixes，適應廣泛的DNA模板和性能需求。
準備好您的血液樣品，來場PCR決賽吧!!
1. Whole blood samples containing sodium citrate, sodium EDTA, potassium EDTA, or sodium heparin are recommended.
2. Blood volume higher than 10 % (v/v) would increase the difficulties in recovering aqueous supernatant from blood cell debris that remains after PCR.
3. Dry blood stored on Guthrie cards or 903 cards (Whatman, NJ) can be used directly by adding 1 mm punch disc to PCR reaction
4. For blood stored on FTA cards (Whatman, NJ), first incubate a 1 mm punch disc in 50 μl of water at 55°C for 5 minutes, then discard the water and use the disc directly in PCR reaction.
6. Up to 10% (v/v) DMSO can be used for high-GC targets.
| Product Name | Size | Cat. No. | ||
|---|---|---|---|---|
| 2X PCR Bestaq™ MasterMix | 5.0 ml (200 Rxns) | G464 | ||
| 2X PCR Bestaq™ MasterMix with dye | 5.0 ml (200 Rxns) | G464-dye | ||
| 2X PCR Taq MasterMix | 5.0 ml (200 Rxns) | G013 | ||
| 2X PCR Taq MasterMix with dye | 5.0 ml (200 Rxns) | G013-dye | ||
| 2X PCR Taq Plus MasterMix | 5.0 ml (200 Rxns) | G014 | ||
| 2X PCR Taq Plus MasterMix with dye | 5.0 ml (200 Rxns) | G014-dye | ||
| Bloodirect 2X PCR MasterMix | 5.0 ml (200 Rxns) | G465 | ||
| 2X PCR Precision™ MasterMix | 5.0 ml (200 Rxns) | G124 | ||
| 2X PCR Precision™ MasterMix with Dye | 5.0 ml (200 Rxns) | G124-dye | ||
| 2X PCR TaqFast MasterMix | 5.0 ml (200 Rxns) | G280 | ||
| 2X PCR TaqFast MasterMix with Dye | 5.0 ml (200 Rxns) | G280-dye | ||
| Safe-Green™ 2X PCR Taq MasterMix | 5.0 ml (200 Rxns) | G472 | ||
| Safe-Green™ 2X PCR Bestaq™ MasterMix | 5.0 ml (200 Rxns) | G478 |
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